The cryo-replicator has 96 spring-loaded pins and allows the simultaneous sampling of 96 frozen glycerol stocks (-80°C) without thawing the remaining glycerol culture. Since the “mother-cultures” remain frozen, the viability (number of CFU’s sampled) is not affected by repeated sampling. Per sampling, less than 0.5 µl is removed from each stock culture so that one master plate can be used for several hundreds of times. The replicator has 45 mm long pins and can also be used for the purpose of transferring 96 colonies simultaneously into a deepwell plate containing liquid growth medium.  

Further information:

Links:

microtiter plate lid

microtiter plate clamps

Main features: 

• Spring-loaded replicator for the simultaneous and reproducible sampling of 
  96 frozen glycerol stocks
• A single master plate can be used more than 100 times
• Sandwich covers convert all 96 wells of a deepwell plate into individual
   “mini-reactors” with identical properties
• Oxygen transfer rates and KLA values during orbital shaking similar
  to those in Erlenmeyer flasks
• No cross-contamination
• Low and uniform evaporation rates
• Various aerobic strains grow to cell densities of 10 g dry wt l-1 
• Designed for use with heterogeneous strain collections of aerobic
  bacteria and streptomycetes, but also applicable for clonal libraries
  in E. coli or yeasts

 Examples: