BPM-60
RAMOS
SFR
CITSens Bio
FeedBeads
FlowCon 2/3/4
Duetz-System
COSBIOS
Duetz-System
Product
Cultivation of bacterial strains, mutants and clone libraries
Description
The Duetz-System is an integrated, easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, and mutant banks in 96-well microtiter plates.
The system enables a single person to grow and test thousands of strains simultaneously without excessive repetitive handling: all handling is performed in parallel for sets of 96 strains. No robotic equipment is required. The system was originally developed and validated at the Institute of Biotechnology of the ETH Zurich.
Performance characteristics
| + An integrated, easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks and cell lines. |
| + 4 Main components: Cryo-replicator, Cryo-replicator press, Sandwich cover, Cover clamps |
| + Cost effective alternative to robot systems |
| + Cultivation of thousands of strains |
| + Same quality of growth as in Erlenmeyer flasks in terms of oxygen transfer rates and reproducibility |
Main components
| Cryo-replicator |
A spring-loaded replicator for the simultaneous and reproducible sampling of 96 frozen glycerol stocks, without melting the remaining culture. |
| Cryo-replicator press |
A guiding system that ensures an accurate vertical movement during the replication of sets of strains. |
| Sandwich covers |
Converts all 96 wells of a square deepwell plate into individual"mini-reactors" (no well-to-well variations) suitable for high-frequency orbital shaking, at high oxygen transfer rates, in the absence of well-to-well variations. |
| Cover clamps |
Secure that the deepwell plate and its sandwich cover are clamped together tightly, thereby preventing well-to-well contamination. |
Complete Duetz-System
Sandwich cover
Holders for microtiter plates
Application fields
Cultivation of bacterial strains, mutants and clone libraries
Application fields
| + High-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous and homogeneous culture collections) |
| + Screening and distribution of clone libraries in E. coli or yeasts |
| + High-throughput screening for high-activity or high-productivity mutants |
| + Comparative studies of e.g. clinical isolates or medium optimization |