kuhnerlife.com

Visit our on kuhnerlife.com:

Read interesting stories about Kuhner life, our partners and projects we support.

EPFL-table

EPFL-table for
50ml bioreactors

Touchscreen

Touchscreen with Kuhner Insight Software for simple operation, monitoring and programming of incubator shakers.

ISF4-X (Climo-Shaker)

Product

Large incubator shaker with a minimal footprint and up to five independent shaker units

Short description

The Kuhner ISF4-X incubator shaker offers large shaking capacity with temperature, humidity and CO2 control all available. The shaker cabinet can incorporate four or even five independent shaking units which are height adjustable. The shaking diameter and speed of each unit can be individually selected. Standard diameters are 12.5, 25 and 50 mm. Other shaking diameters are possible.

Performance characteristics

 
 
+ All stainless steel interior
+ Large shaking capacity
+ Easy access to contents
+ Each shaking unit has its own direct drive
+ Door heating, with controlled humidity

Guaranteed quality

When designing the ISF4-X special emphasis was placed on reliability and durability. The high quality of Kuhner shaking machines is backed with a five year warranty.

Safety and convenience

The large door opening of the ISF4-X allows easy access to the shake flasks inside. The doors are fitted with a safety switch to ensure shaking stops when the doors are opened. 

The large windows allow a clear view of incubator’s contents. The entire ISF4-X chamber is clad in stainless steel making sterilisation with chemicals possible.

Energy saving operation

The highly efficient direct drive system means economic low power consumption and the cooling system also operates with energy efficiency.

Electrical protection

Automatic supervision of the mains voltage protects the shaker and in the event of a voltage spike the unit disconnects from the mains. The machine resumes operation at the set values once power is restored.

Technical data

Large incubator shaker with a minimal footprint and up to five independent shaker units

Technical data

  SMX1600 SMX1601 SMX1603
  SMX1600C* SMX1601C* SMX1603C*
Cooling no yes yes
Humidity control no no yes
Temperature minimum ambient + 10°C ambient - 15°C
(- 10°C)*
ambient - 15°C
(- 10°C)*
Temperature maximum 80°C (60°C)* 80°C (60°C)* 80°C (60°C)*
Humidity maximum - - 85 % r.h.
Power consumption < 1700W < 2000W < 2600W

* incubator shaker optimised for cell cultivation

Machine

   
Gas volume 1272 l
Weight (without SF-X) 520 kg
Weight with 4xSF-X 720 kg
Illumination 2 fl lamps
Operation menu in German, French, English, Italian, Spanish
Interface, standard CAN-Bus
Interface, optional USB, Ethernet, digital, analogue
Ambient temperature 10°C up to 35°C

Dimensions (in mm)

 

Top

2x cable feedthroughs with PG 29 (ID 30mm)

Temperature

   
Setting, digital 0.1°C
Accuracy, absolute ± 0.30°C (37°C)
Principle of sensor Pt-100
Power of heating 1000W
Power of cooling 250…420W
Air circulation 700m3/h

Humidity

  (SMX1603)
max. at 25..55°C 85% r.h.
Setting, digital 1% r.h.
Accuracy, absolute ± 2% r.h.
Principle of sensor capacitive
Water refill automatic
Water heater 300W
Door heater 220W

CO2

  (SMX1034)
Principle of sensor Infrared, NDIR
Measuring range 0...20% CO2
Setting, digital 0.1%
Accuracy, absolute
(including non-linearity, calibration uncertainty, repeatability)
± 0.40% at 5% CO2
Temperature range 5...60°C
CO2-supply max. 2 bar overpressure

O2

  (SMX1738)
O2 sensor zirconium oxide
Measuring range approximately 0% up to ambient O2 (20.95% at dry air)
Accuracy, absolute
±0.40% O2 at 5% O2
O2 sensor stability
±0.5% full scale/year
Setting, digital ± 0.1%
O2 control PID, digital
Temperature range -10...80°C
N2-supply max. 0.5...0.8 bar overpressure

Mains connection

   
SMX1021: 220 - 240 V / 50-60 Hz
SMX1022: 190 - 210 V / 50-60 Hz

Shaking unit SF-X

  (SMX1610)
Tray, size F (800x420mm)
Loading, maximum 25kg
Setting, digital 1 rpm
Accuracy, absolute ± 0.1 rpm
Timer 1s ... 999h
Acceleration controlled
Active brake adjustable
Stop on position adjustable

Shaking motion

  Speed
* orbital, Ø 12.5mm 20...500 rpm
* orbital, Ø 25.0mm 20...400 rpm
* orbital, Ø 50.0mm 20...300 rpm
* linear, Ø 12.5mm 20...400 rpm
* linear, Ø 25.0mm 20...300 rpm
* linear, Ø 50.0 mm 20…200 rpm
* can be changed  

Options

Large incubator shaker with a minimal footprint and up to five independent shaker units

Cooling

All Kuhner incubator shakers can be fitted with cooling. The refrigeration system is regulated by a fully digital PID control ensuring minimal energy consumption. The cooling is switched on only when required as determined by the chamber temperature and set point.

Humidity control

Humidity control is an important factor in successful fermentation. Evaporation from microtiter plates, or when cultivating in flasks for long periods (e.g. cell cultures), can be significantly reduced with humidification.

CO2 control

CO2 control is essential when working with mammalian cell cultures. Kuhner was the first company to manufacture and supply incubator shakers with CO2 control more than ten years ago and continues to offer this option.

Pull-out table

The pull-out table is designed for the ISF1-X and ISF4-X incubator shakers as well as the Rack System. This remarkable device makes loading and unloading trays much easier.

 

Dual table

The dual table is an easy and economical way of doubling the shaking capacity. The dual shaking table consists of two levels. Each level will accept an F size tray. However, with the dual table the shaking speed is limited to a maximum of 200 rpm.

 

Illumination unit for photosynthesis (LED)

The ceiling of any Kuhner incubator shaker can be fitted with LED modules for the cultivation of phototrophic organisms. The control module allows full programming of night/day cycles and variable light intensity.

 

UV lamp

As in a biological safety cabinet, the chamber of a Kuhner incubator shaker can also be sterilized with an integrated UV lamp. The lamp is situated to maximise irradiation of the chamber and shaking table. The UV lamp has a clearly marked external switch and when the door is opened, the UV-lighting is automatically switched off.

 

Additional integrated UV lamp

In the space between the incubator chamber and the rear outer wall of the ISF4-X, a UV lamp can be mounted. This can be used while shaking to decrease the number of germs in the circulated air.

Black window

Available for light sensitive medium or organisms. Any Kuhner incubator shaker can be delivered with blackened windows to prevent unwanted daylight or UV radiation inside the incubator.

Touchscreen

This option includes a 9-inch touchscreen with our Kuhner Insight Software. The touchscreen has been designed for simple operation, monitoring and programming. It can also be retrofitted to existing incubator shakers.

Perspex cover

To prevent accidental operation of the controllers, the panel can be protected by a perspex cover.

BPM-60

BPM-60 (Bioprocess Monitoring) is a non-invasive, online measurement of dissolved oxygen and pH in an Erlenmeyer flask.

 

Continuous, recording of dissolved oxygen and pH

Dissolved oxygen and pH can be easily measured online using the BPM-60. The system allows for one or both parameters to be recorded in the same flask. The values of dissolved oxygen and/or pH can be monitored simultaneously in eight different flasks.

 

The continuous data logging, compared to conventional sample taking, is automatic and consistent. The recorded data can be analysed to identify oxygen limitation or inhibition and therefore optimize yields in shaken flasks.

Furthermore, the BPM-60 assists in the production of reproducible starter cultures for use in fermenters (see PAT-initiative of the FDA).

 

Not on battery mode

An integrated socket (TabCom option) at the front of the shaking table makes simple data communication and power supply possible without the risk of wiring breaking. With this technology no battery is required.

 

Kuhner Insight software for data logging

The Kuhner Insight software is capable of data recording. In addition to logging dissolved oxygen and pH it is also possible to record all other shaker parameters such as shaking speed and CO2 concentration. A full audit trail is also stored for each shaker connected.

 

  • Further information can be found under add-ons.

TabCom

The TabCom option from Kuhner consists of a cable for power and data with the connection port integrated in the shaking table (see pictures below). A cable guide prevents the cable breaking and ensures secure data recording. Online measuring technologies offered by Kuhner that use TabCom include BPM-60 (pH, dissolved oxygen) and RAMOS (OTR, CTR). The flexibility of TabCom means other measurement systems can be easily integrated.


Order number

SMX1612A tl_files/kuhner/product/shaker/options/Port_TabCom.png

TabCom Option for
standard shaking unit


CAN-Bus + 24 V power supply
at the shaking table

SMX1612B tl_files/kuhner/product/shaker/options/TabCom_pull out table_web.png

TabCom Option for units
with pull-out table


CAN-Bus + 24 V power supply
at the shaking table

 

IQ-OQ Documentation

IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.

Accessories

Large incubator shaker with a minimal footprint and up to five independent shaker units

Universal tray FU

Order number: SM3003


The universal tray can be fitted with clamps for Erlenmeyer flasks, test tube
holders, microtiter plates, or custom made holders to suit your requirements.
Rearranging these clamps and holders is quick and simple.

 

 

ISF4-X with Universal trays FU,
fitted with Ultra Yield flasks (2500 mL) from Thomson

 

A FU-tray can be fitted with 8x clamps for Ultra Yield flasks (2500 mL) from Thomson.

 

 

Order number Description
SM312500U Clamp U-2500 for Ultra Yield flask from Thomson

 

F-tray with fixed clamps

The clamps are riveted onto these trays and cannot be removed. They offer maximum stability and will often hold a larger number of flasks than universal trays. These trays are available in a variety of flask clamp sizes from 25 to 6000ml. 

Order number

  Description Number of clamps
SM330025 F-25 ml 153
SM330050 F-50 ml 100
SM330100 F-100 ml 74
SM330125 F-125 ml 60
SM330150 F-150 ml 60
SM330200 F-200 ml 40
SM330250 F-250 ml 40
SM330300 F-300 ml 30
SM330500 F-500 ml 26
SM331000 F-1000 ml 16
SM331500 F-1500 ml 12
SM332000 F-2000 ml 9
SM332500U F-2500U 8
SM333000 F-3000 ml 8
SM334000 F-4000 ml 5
SM335000 F-5000 ml 4
SM336000 F-6000 ml 3

F-trays for microtiter plates

Kuhner has designed trays specifically for microtiter plates. Handles at the front of the tray open and close the special clamping mechanism allowing plates to be loaded and unloaded. Trays of different heights (22, 47 and 77mm) allow the
stacking of up to four microtiter plates. The tray is suitable for all types of plates (deep well, low well, 24, 48 and 96 well).

 


Order number Description Number of Microtiter plates
SM3503.22 F-MT.22 24 - 48
SM3503.47 F-MT.47 24 - 96
SM3503.77 F-MT.77 24 - 144

F-tray with pins

Kuhner has designed a new F-size tray (800 x 420mm) which is ideal for cell culture. The F-V5000/7 tray allows parallel shaking of 7 x 3L Corning Fernbach culture flasks, 7 x 5L Corning Erlenmeyer flasks or 7 x 5L Thomson Optimum Growth flasks. Flasks are held in position by pins and are easily placed on the tray and removed just as quickly.

This tray has been designed for easy cleaning and efficient handling of samples.

 

 

Order number

  Description
SM335000S F-V5000/7
F-tray with pins for 7 flasks:
- 3L or 5L Corning
- 5L Thomson

F-tray with rubber mat

Order number: SM3603


The whole tray is covered with a rubber mat and is particularly suitable for
shaking containers at low speed (e.g. blood bags).

 

F-tray with sticky strips

Order number: SMX350001


A tray fitted with sticky strips is especially useful for flasks shaken at low speeds (up to 200rpm). The flask is placed directly on the sticky strip without the need for any other support. Flasks of all sizes can be used. Sticky strips are available individually or in sets and can also be supplied already fitted to a tray. The F-size tray accepts eight strips.

 

F-tray with support bars

Order number: SM4130.6


This tray offers an alternative to both clamps and sticky mats and is ideal for tall containers. The tray is covered with a rubber mat and has an upright at each corner. The F-tray is fitted with six cross supports. These support bars securely clamp containers.

 

F-tray for separating vessels

Kuhner has also designed a special tray for shaking separating vessels. This very flexible tray can take vessels of various sizes. It is also possible to add a second layer and double the capacity. Movable retaining rods allow vessels to be installed easily.

Order number

  Description Number of vessels
SM430250 FS-250 10
SM4302502 FS-250/2 20 (2 layers)
SM430500 FS-500 8
SM4305002 FS-500/2 16 (2 layers)
SM431000 FS-1000 6
SM4310002 FS-1000/2 12 (2 layers)

F-tray for centrifuge tubes

Order number: SM3805


The use of centrifuge tubes (e.g. 50ml Falcon tubes and TPP 50ml) in cell culture screening is well established. This F-tray has a capacity of 120 x 50 ml centrifuge tubes (5 racks of 24).

Holders for 600ml TPP tubes are also available.

 

Shelf

Order number: SMX1672


The ISF4-X incubator shakers can be fitted with a shelf allowing cultivation in petri dishes. One or two shelves can be placed above the shaking table and fixed to the chamber walls with four screws.

Kuhner Insight Software

The user-friendly Kuhner Insight software was developed for supervision, monitoring, and recording of data. The software is easy to operate

Interfaces

Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces.

FAQ

Large incubator shaker with a minimal footprint and up to five independent shaker units

? Is linear shaking available?
! Yes.
? Is it possible to alter the height of the shaking units in an ISF4-X?
! Yes, there are 37 possible positions.
? How many shaking units will fit in an ISF4-X?
! It depends on the height of the vessels you are shaking. Please contact Kuhner or your local distributor for further information.
? Can the shaking diameter and speed of each shaking unit be set individually?
! Yes.
? Is IQ-OQ documentation available for the shakers?
! Yes.
? Can the machine be used in the GMP sector?
! Yes.